The Advent of Semi-Elective Lung Transplantation—Prolonged Static Cold Storage at 10°C
- 1Department of Thoracic Surgery, Medical University of Vienna, Vienna, Austria
- 2Toronto Lung Transplant Program, Division of Thoracic Surgery, University Health Network, University of Toronto, Toronto, ON, Canada
Since the early days of clinical lung transplantation the preservation of donor organs has become a fairly standardized procedure and most centers do follow similar processes. This includes the use of low-potassium high dextran flush solutions and static cold storage (SCS) in a cooler filled with ice. Depending on the length of SCS, organs usually arrive at the recipient hospital at a temperature of 0°C–4°C. The question of the optimal storage temperature for donor lung preservation has been revisited as data from large animal experiments demonstrated that organs stored at 10°C experience less mitochondrial damage. Thus, prolonged cold ischemic times can be better tolerated at 10°C—even in pre-damaged organs. The clinical applicability of these findings was demonstrated in an international multi-center observational study including three high-volume lung transplant centers. Total clinical preservation times of up to 24 hrs have been successfully achieved in organs stored at 10°C without hampering primary organ function and short-term outcomes. Currently, a randomized-controlled trial (RCT) is recruiting patients with the aim to compare standard SCS on ice with prolonged SCS protocol at 10°C. If, as anticipated, this RCT confirms data from previous studies, lung transplantation could indeed become a semi-elective procedure.
Introduction
Since the initiation of clinical lung transplantation, the procedure has always been defined as an emergent or acute operation, which requires teams to adhere to a strict timeline to minimize ischemic injury to the graft. There is currently a general consensus within the lung transplant community, that the length of graft preservation clinically should not exceed 6–8 h. This practice is reflected in large database analyses including the International Society for Heart and Lung Transplantation (ISHLT) Registry data. These studies have uniformly shown that short-term mortality increases with longer preservation time, with 30-day survival being significantly worse for ischemic times greater than 6 h [1]. Storage on ice has been considered the standard of care for preservation of donor lungs (and all organs for that matter) and has been implemented as such in lung transplant centers around the world. Storage on ice is intended to provide storage temperatures ranging between 0°C and 4°C, which is empirically considered clinically safe.
Current Evidence in Donor Lung Preservation
The current practice in lung transplantation—including optimal donor lung preservation–is mostly based on empirical clinical experience and expert consensus. Due to the low number of procedures performed per year, it has often been considered difficult to provide robust data in the field of lung transplantation. Moreover, the significant heterogeneity among patients and center practice has represented a major hurdle to design multicenter prospective clinical trials. To the best of our knowledge, only two randomized controlled trials have been published in the field of donor lung preservation. Both of them compared an ex-vivo lung perfusion (EVLP) protocol with SCS. The INSPIRE trial [2] tested the Organ Care System in “standard” donor lungs. Before that, a single-center RCT was conducted by the Vienna Lung Transplant Program which compared the Toronto EVLP protocol to SCS, also in standard donor lungs [3]. Both trials showed comparable results of EVLP with the “simpler” and “cheaper” static preservation on ice. Therefore, EVLP did not replace SCS, but was introduced to clinical practice as a tool to evaluate lungs with a questionable quality and to optimize marginal donor lungs [4–8].
Lung Preservation at 10°C
As early as 30 years ago, attempts were made to understand the effects of temperature on graft function and to optimize the temperature during donor lung preservation [9]. Subsequently this was confirmed in a study by Kayano et al., using a rat model, that reported an optimal storage temperature of 10°C [10]. Similar findings were observed in a large animal (canine) lung transplant study, published in 1992, comparing three different preservation temperatures [11]. Once again, lungs preserved at 10° degrees showed better oxygenation and decreased pulmonary vascular resistance. However, because of the lack of ability to accurately maintain organs at 10°C at the time (and incomplete understanding of the underlying biologic mechanisms), clinical concern that a temperature increase above this 10°C threshold could have deleterious effects on the lungs, the lower, more convenient target value of 4°C was widely adopted to provide a safety margin. As such, SCS on ice came to be defined as the “standard of care.”
One of the main disadvantages of SCS on ice is that the true temperature of the donor lung can significantly diverge from 4°C. A thermographic evaluation of donor lungs transported on ice showed that the surface temperature was non-homogenous and depending on preservation times ranged from 0.2°C to 10.6°C [12]. Consequently, temperature-controlled preservation devices, which use more accurate cool packs instead of ice cubes, have been developed.
Rationale
The rationale underlying hypothermic organ storage is to reduce cellular metabolism, and thus maintain viability during the storage time with limited oxygen and nutrients. Since most of the deleterious effects of hypoxia are caused by simple biochemical reactions, it seemed reasonable to reduce the temperature close to 4°C in order to decrease enzyme activity in the donor organ. However, because this approach is non-selective, vital enzymes, such as Na+/K+ ATPase, are also affected in their function, leading to an ionic imbalance that can result in cell edema and damage [13]. In addition, intracellular calcium accumulation induces further cellular damage and the formation of reactive oxygen species can be promoted during cold ischemia [14]. In recent years, efforts have been made to optimize preservation strategies by revisiting the topic of optimal storage temperature of donor lungs. These efforts aimed to extend preservation times in order to increase the donor pool, optimize the immunological matching between donor and recipient and transition organ implantation from an urgent to a semi-elective procedure.
Pre-Clinical Studies
First, the feasibility of prolonged donor lung storage at 10°C was assessed in a large animal model. Porcine lungs stored for 36 h at 10°C showed lower airway pressures, had a better lung compliance and improved oxygenation after implantation as compared to lungs stored conventionally on ice [15]. Importantly, markers of mitochondrial injury were found to be lower in the study group, which provided a mechanistic insight into the benefit of 10°C storage (Figure 1). In a subsequent study, a possible recovery or regenerative effect of 10°C preservation was tested in a model of gastric acid aspiration injury [16]. Moderate lung damage was induced by intrabronchial instillation of gastric juice. Injured donor lungs were harvested and randomly assigned to storage for 12 h on ice or at 10°C. A third group consisted of immediate transplantation after only a short period of SCS on ice. A left single lung transplant was performed, followed by a 4-hour functional assessment. During reperfusion, lungs stored at 10°C showed significantly better oxygenation. Moreover, they had lower tissue levels of IL-1β after reperfusion, histologic evaluation demonstrated lower acute lung injury scores and significantly less apoptosis in the 10°C group. In all measured parameters, storage of lungs at 10°C for 12 h was associated with improved graft quality, even when compared to minimal cold ischemia on ice.
![www.frontiersin.org](https://www.frontierspartnerships.org/files/Articles/12310/ti-37-12310-HTML/image_t/ti-37-12310-g001.gif)
FIGURE 1. Levels of metabolites relevant for mitochondrial oxidative protection are displayed in (A). (B) shows a scheme of how the tricarboxylic acid (TCA) cycle impacts mitochodrial health.
Clinical Studies
These preclinical data were then translated to the clinical setting and a multicenter non-randomized clinical trial was designed. Three high-volume lung transplant centers—Toronto, Vienna, Madrid—recruited patients in a safety and feasibility study [17]. In this study, grafts from donors with cross-clamp times between 6:00 PM and 4:00 AM had an earliest possible implantation start time of 6:00 AM. Lungs were retrieved and transported using a traditional ice cooler. Upon arrival at the transplant hospital, the lungs were transferred to a temperature-controlled 10°C incubator (MYTEMP™65HC, Benchmark Scientific) and were stored until implantation. The primary outcome of this study was the incidence of Primary Graft Dysfunction (PGD) grade 3 at 72 h. Results were compared to a contemporary cohort of recipients, who received donor lungs that had been preserved by SCS on ice, using propensity score matching at a 1:2 ratio. Seventy patients were included in the study arm. Mean cold static preservation was significantly longer in the 10°C study group vs. matched controls for both the first and second implanted lung (Table 1). PGD grade 3 at 72 h was 5.7% in the study group vs. 9.3% in matched controls (p = 0.39). There were no differences in the need for post-op ECMO, median ICU length of stay (LOS) or median hospital LOS between the two groups. Also, one-year survival was similar between the two groups (p = 0.37) with a median follow-up time of 336 days [17].
![www.frontiersin.org](https://www.frontierspartnerships.org/files/Articles/12310/ti-37-12310-HTML/image_t/ti-37-12310-t001.gif)
TABLE 1. Post-transplant outcomes of pilot, prospective, multi-center, non-randomized clinical trial, adapted from Ali et.al. permission to reprint obtained [17].
Based on the above findings, a multicenter, prospective, randomized-controlled trial was designed, to which recruitment officially started in May 2023 (NCT05898776). This study, involving 15 transplant centers worldwide, is designed as a non-inferiority study that will compare an extended preservation period (time from donor aortic cross-clamp to anesthesia start in the recipient hospital) of up to 12 hrs using a portable 10°C cooler (XPort, Traferox, Toronto, Canada) to conventional preservation (SCS on ice; time from donor aortic cross-clamp to anesthesia start in the recipient hospital of up to 6h). The results of this RCT will hopefully provide the evidence for changing the standard practice of donor lung preservation, which will in turn lead to significant flexibility in clinical lung preservation times.
Advantages of Prolonged Storage at 10°C
Avoid Night Time Transplantation
Multi-organ donation and the subsequent need to coordinate several organ procurement and implantation teams has shaped transplant medicine into an acute and challenging discipline. As all donor organs have different tolerances to cold ischemia and operating room capacity is often limited at procurement sites, multiorgan procurement is often performed in the evening or night time. As a result, implantation teams are often required to perform complex and exhausting procedures in critical recipients during night time hours. Of interest, several studies in the field of transplantation have linked night time procedures with worse clinical outcomes. It has been demonstrated that night time lung transplant recipients had a higher rate of postoperative complications than daytime recipients [18]. Similarly, it has been shown that night time liver transplant recipients have a 2-fold increased short-term mortality [19]. The possibility to prolong preservation times and thus render lung transplantation into a semi-elective and day time procedure has the potential to improve patient outcomes and as quality of life for transplant professionals, which would profoundly change current practice.
Postpone Implantation for Logistic Reasons
The limited preservation time of grafts can create significant logistic problems for lung transplant centers. In this light, extending the time window of implantation by being able to store grafts at 10°C increases flexibility and offers several advantages: (i) scheduled elective cases can be finished by deliberately moving the implantation to the afternoon; (ii) surgically complex recipients can be transplanted during the day when the team can perform at its best, or the most experienced team is available; (iii) small and medium-sized lung transplant centers may be able to accept concurrent donor offers and safely implant the organs one after another. These logistical advantage has already in fact been successfully described by the Madrid transplant program, which accepted two parallel donors and postponed the most complex of the two cases until daytime, thus avoiding parallel surgery in the operating room overnight [20]. In addition, prolonged organ storage provides the opportunity to optimize a recipient preoperatively. As highly sensitized recipients are increasingly accepted by lung transplant programs, preoperative sensitization protocols including immunoadsorption or plasmapheresis could potentially be performed without time constraints. Finally, higher logistical flexibility can have a direct impact on the quality of life of transplant candidates. Patients on the waiting list who reside in remote areas may have the opportunity to remain in their hometown despite longer transport times without the need to relocate near the transplant center—a major social and economic advantage for them.
Broader Geographic Distribution of Donor Organs
The shortage of donor lungs remains one of the biggest hurdles in clinical lung transplant practice. In Europe, North America and around the world, a large number of optimal donor offers are often declined, simply due to the long transport times involved. An easy-to-use, cost effective and reusable temperature-controlled device that ensures organ storage at 10°C for an extended period of time could fundamentally change our current practice. Based on the scientific evidence presented above, such a device could safely extend the geographic boundaries and facilitate a broader organ sharing.
Another interesting concept that is linked with increased preservation times, is fostering environmentally friendly transportation modes. Currently, the majority of donor lungs are transported by charter airplane flights [21]. Especially in Europe, where travelling distances are comparably short, most organs could be transported by commercial flights, car or train in the near future. This has the potential to significantly improve the carbon footprint of organ procurement and decrease costs [22].
Reduce the Number of “False-Calls” for Recipients
“Dry runs” are common in lung transplantation with rates of up to 40 percent being reported in DCD donors [23]. Currently, recipients are immediately informed when an organ has been allocated to them and they usually have to promptly come to the hospital. “Dry runs” pose an enormous emotional burden to recipients and their families. Such false calls could be completely avoided by prolonged 10°C preservation as patients would only be informed when a lung has been finally accepted for transplantation.
Future Directions of Organ Preservation
Donor lung preservation is one of the most studied topics of clinical lung transplantation. Most of recent research aims to either prolong preservation times or improve organ quality. We foresee and increasing role of ex-vivo lung perfusion with a constant improvement in perfusion and ventilation strategies. Several attempts have recently been made to optimize perfusion solutions in order to prolong EVLP times, i.e., adding nutrients or maintaining perfusate osmolality and pH [24, 25]. Another interesting concept might be the use of hypothermic ex-vivo lung perfusion [26]. HOPE is already routinely used in liver and kidney transplantation [27]. In addition, it has recently also been successfully tested in heart transplantation [28].
The other main perspective of ex-vivo lung perfusion is its role as a repair platform where reversible conditions of the donor organ can be treated. Promising data from animal study are already available. EVLP has been successfully used to reduce bacterial load [29], to reverse inflammatory damage related to aspiration [30], and to clear lungs from infections such as HCV [31]. In a subsequent step EVLP could be used to manipulate donor lungs and render them into ‘super organs’. Modulating immunogenicity by inducing IL-10 overexpression or cleaving surface antigens are possible approaches.
Conclusion
Clinical donor lung preservation will significantly change in the future. Storage of grafts at 10°C will have a considerable impact on transplant programs around the world by extending acceptable and safe preservation times. This will shift in our clinical practice towards an unprecedented semi-elective transplantation practice with numerous beneficial effects.
Author Contributions
KH and AB wrote the manuscript. All authors contributed to the article and approved the submitted version.
Conflict of Interest
SK serves as Chief Medical Officer of Traferox Technologies, SK and MC receive personal fees from Lung Bioengineering and Traferox Technologies. SK and MC are shareholders of Traferox Technologies. SK and MC fully adhere to policies at University Health Network that ensure academic integrity and management of potential interest.
The Remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
References
1. Chambers, DC, Yusen, RD, Cherikh, WS, Goldfarb, SB, Kucheryavaya, AY, Khusch, K, et al. The Registry of the International Society for Heart and Lung Transplantation: Thirty-Fourth Adult Lung and Heart-Lung Transplantation Report-2017; Focus Theme: Allograft Ischemic Time. J Heart Lung Transpl (2017) 36:1047–59. doi:10.1016/j.healun.2017.07.016
2. Warnecke, G, Van Raemdonck, D, Smith, MA, Massard, G, Kukreja, J, Rea, F, et al. Normothermic Ex-Vivo Preservation With the Portable Organ Care System Lung Device for Bilateral Lung Transplantation (INSPIRE): A Randomised, Open-Label, Non-Inferiority, Phase 3 Study. Lancet Respir Med (2018) 6:357–67. doi:10.1016/S2213-2600(18)30136-X
3. Slama, A, Schillab, L, Barta, M, Benedek, A, Mitterbauer, A, Hoetzenecker, K, et al. Standard Donor Lung Procurement With Normothermic Ex Vivo Lung Perfusion: A Prospective Randomized Clinical Trial. J Heart Lung Transpl (2017) 36:744–53. doi:10.1016/j.healun.2017.02.011
4. Dunne, B, Pozniak, J, Campo-Canaveral de la Cruz, JL, Lemaitre, P, Ma, J, Pierre, AF, et al. Ex Vivo Lung Evaluation of Single Donor Lungs When the Contralateral Lung Is Rejected Increases Safe Use. J Thorac Cardiovasc Surg (2023) 165:526–31.e1. doi:10.1016/j.jtcvs.2022.03.012
5. Di Nardo, M, Del Sorbo, L, Sage, A, Ma, J, Liu, M, Yeung, JC, et al. Predicting Donor Lung Acceptance for Transplant During Ex Vivo Lung Perfusion: The Ex Vivo Lung PerfusIon pREdiction (EXPIRE). Am J Transpl (2021) 21:3704–13. doi:10.1111/ajt.16616
6. Divithotawela, C, Cypel, M, Martinu, T, Singer, LG, Binnie, M, Chow, CW, et al. Long-Term Outcomes of Lung Transplant With Ex Vivo Lung Perfusion. JAMA Surg (2019) 154:1143–50. doi:10.1001/jamasurg.2019.4079
7. Mallea, JM, Hartwig, MG, Keller, CA, Kon, Z, Iii, RNP, Erasmus, DB, et al. Remote Ex Vivo Lung Perfusion at a Centralized Evaluation Facility. J Heart Lung Transpl (2022) 41:1700–11. doi:10.1016/j.healun.2022.09.006
8. Okamoto, T, Omara, M, Ahmad, U, Yun, J, Bribriesco, A, Unai, S, et al. Utilization of Marginal Lung Donors With Low PaO(2)/FiO(2) Ratio and High Body Mass Index. Ann Thorac Surg (2020) 109:1663–9. doi:10.1016/j.athoracsur.2019.12.072
9. Wang, LS, Yoshikawa, K, Miyoshi, S, Nakamoto, K, Hsieh, CM, Yamazaki, F, et al. The Effect of Ischemic Time and Temperature on Lung Preservation in a Simple Ex Vivo Rabbit Model Used for Functional Assessment. J Thorac Cardiovasc Surg (1989) 98:333–42. doi:10.1016/s0022-5223(19)34378-8
10. Kayano, K, Toda, K, Naka, Y, and Pinsky, DJ. Identification of Optimal Conditions for Lung Graft Storage With Euro-Collins Solution by Use of a Rat Orthotopic Lung Transplant Model. Circulation (1999) 100:Ii257–61. doi:10.1161/01.cir.100.suppl_2.ii-257
11. Date, H, Lima, O, Matsumura, A, Tsuji, H, d'Avignon, DA, and Cooper, JD. In a Canine Model, Lung Preservation at 10 Degrees C Is Superior to That at 4 Degrees C. A Comparison of Two Preservation Temperatures on Lung Function and on Adenosine Triphosphate Level Measured by Phosphorus 31-Nuclear Magnetic Resonance. J Thorac Cardiovasc Surg (1992) 103:773–80.
12. Ahmed, HH, Alayyar, M, Jothidasan, A, Husain, M, Zeschky, C, Umakumar, K, et al. Thermographic Imaging of Lungs Procured and Transported on Ice. J Heart Lung Transplant (2022) 41:S253. doi:10.1016/j.healun.2022.01.1778
13. Boutilier, RG. Mechanisms of Cell Survival in Hypoxia and Hypothermia. J Exp Biol (2001) 204:3171–81. doi:10.1242/jeb.204.18.3171
14. Zhao, G, Al-Mehdi, AB, and Fisher, AB. Anoxia-Reoxygenation Versus Ischemia in Isolated Rat Lungs. Am J Physiology-Lung Cell Mol Physiol (1997) 273:L1112–7. doi:10.1152/ajplung.1997.273.6.L1112
15. Ali, A, Wang, A, Ribeiro, RVP, Beroncal, EL, Baciu, C, Galasso, M, et al. Static Lung Storage at 10°C Maintains Mitochondrial Health and Preserves Donor Organ Function. Sci Transl Med (2021) 13:eabf7601. doi:10.1126/scitranslmed.abf7601
16. Abdelnour-Berchtold, E, Ali, A, Baciu, C, Beroncal, EL, Wang, A, Hough, O, et al. Evaluation of 10°C as the Optimal Storage Temperature for Aspiration-Injured Donor Lungs in a Large Animal Transplant Model. J Heart Lung Transpl (2022) 41:1679–88. doi:10.1016/j.healun.2022.08.025
17. Ali, A, Hoetzenecker, K, de la Cruz, JLC-C, Schwarz, S, Burturen, MG, Tomlinson, G, et al. Extension of Cold Static Donor Lung Preservation at 10°C. NEJM Evid (2023) 2:EVIDoa2300008. doi:10.1056/EVIDoa2300008
18. Yang, Z, Takahashi, T, Gerull, WD, Hamilton, C, Subramanian, MP, Liu, J, et al. Impact of Nighttime Lung Transplantation on Outcomes and Costs. Ann Thorac Surg (2021) 112:206–13. doi:10.1016/j.athoracsur.2020.07.060
19. Lonze, BE, Parsikia, A, Feyssa, EL, Khanmoradi, K, Araya, VR, Zaki, RF, et al. Operative Start Times and Complications After Liver Transplantation. Am J Transplant (2010) 10:1842–9. doi:10.1111/j.1600-6143.2010.03177.x
20. Gil Barturen, M, Laporta Hernández, R, Romero Berrocal, A, Pérez Redondo, M, Romero Román, A, Crowley Carrasco, S, et al. Donor Lungs Cold Preservation at 10 °C Offers a Potential Logistic Advantage in Lung Transplantation. Cir Esp (Engl Ed (2023) 101:283–6. doi:10.1016/j.cireng.2022.11.005
21. Lehr, CJ, Skeans, MA, Lease, ED, and Valapour, M. Effects of Broader Geographic Distribution of Donor Lungs on Travel Mode and Estimated Costs of Organ Procurement. Am J Transpl (2021) 21:4012–22. doi:10.1111/ajt.16697
22. Wall, AE, Borries, T, Reddy, V, Asrani, SK, Testa, G, and Trotter, J. The Carbon Footprint of Organ Acquisition in the United States. Am J Transpl (2022) 22:3184–5. doi:10.1111/ajt.17196
23. Tsuang, W, and Udeh, B. Improving Access to Lung Transplant Through Donor Strategy: A Decision Analysis. Am J Transpl (2019) 19.
24. Shin, J, Hsin, MK, Baciu, C, Chen, Y, Zamel, R, Machuca, T, et al. Use of Metabolomics to Identify Strategies to Improve and Prolong Ex Vivo Lung Perfusion for Lung Transplants. J Heart Lung Transpl (2021) 40:525–35. doi:10.1016/j.healun.2021.02.002
25. Takahashi, M, Andrew Cheung, HY, Watanabe, T, Zamel, R, Cypel, M, Liu, M, et al. Strategies to Prolong Homeostasis of Ex Vivo Perfused Lungs. J Thorac Cardiovasc Surg (2021) 161:1963–73. doi:10.1016/j.jtcvs.2020.07.104
26. Arni, S, Maeyashiki, T, Citak, N, Opitz, I, and Inci, I. Subnormothermic Ex Vivo Lung Perfusion Temperature Improves Graft Preservation in Lung Transplantation. Cells (2021) 10:748. doi:10.3390/cells10040748
27. Parente, A, Tirotta, F, Pini, A, Eden, J, Dondossola, D, Manzia, TM, et al. Machine Perfusion Techniques for Liver Transplantation - A Meta-Analysis of the First Seven Randomized-Controlled Trials. J Hepatol (2023) 79:1201–13. doi:10.1016/j.jhep.2023.05.027
28. McGiffin, DC, Kure, CE, Macdonald, PS, Jansz, PC, Emmanuel, S, Marasco, SF, et al. Hypothermic Oxygenated Perfusion (HOPE) Safely and Effectively Extends Acceptable Donor Heart Preservation Times: Results of the Australian and New Zealand Trial. J Heart Lung Transpl (2023) S1053-2498:02110–1. doi:10.1016/j.healun.2023.10.020
29. Nakajima, D, Cypel, M, Bonato, R, Machuca, TN, Iskender, I, Hashimoto, K, et al. Ex Vivo Perfusion Treatment of Infection in Human Donor Lungs. Am J Transpl (2016) 16:1229–37. doi:10.1111/ajt.13562
30. Nakajima, D, Liu, M, Ohsumi, A, Kalaf, R, Iskender, I, Hsin, M, et al. Lung Lavage and Surfactant Replacement During Ex Vivo Lung Perfusion for Treatment of Gastric Acid Aspiration-Induced Donor Lung Injury. J Heart Lung Transpl (2017) 36:577–85. doi:10.1016/j.healun.2016.11.010
Keywords: 10°C, lung transplantation, semi-elective, preservation, prolonged storage
Citation: Hoetzenecker K, Benazzo A, Schwarz S, Keshavjee S and Cypel M (2024) The Advent of Semi-Elective Lung Transplantation—Prolonged Static Cold Storage at 10°C. Transpl Int 37:12310. doi: 10.3389/ti.2024.12310
Received: 26 October 2023; Accepted: 09 January 2024;
Published: 22 January 2024.
Copyright © 2024 Hoetzenecker, Benazzo, Schwarz, Keshavjee and Cypel. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: K. Hoetzenecker, konrad.hoetzenecker@meduniwien.ac.at