AUTHOR=Madduri Madhuri , Rudramurthy Shivaprakash M. , Roy Utpal 

TITLE=Two promising natural lipopeptides from Bacillus subtilis effectively induced membrane permeabilization in Candida glabrata

JOURNAL=Acta Biochimica Polonica

VOLUME=71

YEAR=2024

URL=https://www.frontierspartnerships.org/journals/acta-biochimica-polonica/articles/10.3389/abp.2024.11999

DOI=10.3389/abp.2024.11999

ISSN=1734-154X

ABSTRACT=<p><italic>Candida glabrata</italic> is an important opportunistic human pathogen well known to develop resistance to antifungal drugs. Due to their numerous desirable qualities, antimicrobial lipopeptides have gained significant attention as promising candidates for antifungal drugs. In the present study, two bioactive lipopeptides (AF<sub>4</sub> and AF<sub>5</sub> m/z 1071.5 and 1085.5, respectively), coproduced and purified from <italic>Bacillus subtilis</italic> RLID12.1, consist of seven amino acid residues with lipid moieties<italic>.</italic> In our previous studies, the reversed phased-HPLC purified lipopeptides demonstrated broad-spectrum of antifungal activities against over 110 <italic>Candida albicans, Candida</italic> non<italic>-albicans</italic> and mycelial fungi. Two lipopeptides triggered membrane permeabilization of <italic>C. glabrata</italic> cells, as confirmed by propidium iodide-based flow cytometry, with PI uptake up to 99% demonstrating fungicidal effects. Metabolic inactivation in treated cells was confirmed by FUN-1-based confocal microscopy. Together, the results indicate that these lipopeptides have potentials to be developed into a new set of antifungals for combating fungal infections.</p>