AUTHOR=Winnicka Dorota , Skowera Paulina , Stelmach Magdalena , Styka Borys , Lejman Monika TITLE=Application of the FISH method and high-density SNP arrays to assess genetic changes in neuroblastoma—research by one institute JOURNAL=Acta Biochimica Polonica VOLUME=Volume 71 - 2024 YEAR=2024 URL=https://www.frontierspartnerships.org/journals/acta-biochimica-polonica/articles/10.3389/abp.2024.12821 DOI=10.3389/abp.2024.12821 ISSN=1734-154X ABSTRACT=
Neuroblastoma is the most common extracranial solid tumor in children. Amplification of the MYCN gene has been observed in approximately 20%–30% of tumors. It is strongly correlated with advanced-stage disease, rapid tumor progression, resistance to chemotherapy and poor outcomes independent of patient age and stage of advanced disease. MYCN amplification identifies high-risk patients. To assess neuroblastoma tumors with MYCN amplification we used paraffin-embedded tissue sections in 57 patients and intraoperative tumor imprints in 10 patients by fluorescence