AUTHOR=Huang Jie , Wang Mingshu , Cheng Anchun , Tian Bin , Ou Xuming , Wu Ying , Yang Qiao , Sun Di , Zhang Shaqiu , Mao Sai , Zhao Xinxin , Huang Juan , Gao Qun , Zhu Dekang , Jia Renyong , Chen Shun , Liu Mafeng 

TITLE=Duck enteritis virus UL7 is a late gene and the UL7-encoded protein co-localizes with pUL51

JOURNAL=Acta Virologica

VOLUME=68

YEAR=2024

URL=https://www.frontierspartnerships.org/journals/acta-virologica/articles/10.3389/av.2024.12023

DOI=10.3389/av.2024.12023

ISSN=1336-2305

ABSTRACT=<sec><title>Background</title><p>Duck enteritis virus (DEV) belongs to <italic>Alphaherpesvirinae</italic>; little is known about the DEV <italic>UL7</italic> gene and its encoded protein. This study examined the molecular characteristics of DEV pUL7 <italic>in vitro</italic> and determined whether DEV pUL7 co-localizes with pUL51.</p></sec><sec><title>Results</title><p>The results showed that <italic>UL7</italic> can be regarded as a late gene. Moreover, immunofluorescence assay revealed that pUL7 was located around the perinuclear cytoplasmic region and co-localized with pUL51 in the cytoplasm and nucleus after transfection into duck embryo fibroblast cells (DEFs).</p></sec><sec><title>Conclusion</title><p>In conclusion, we identified the molecular characteristics of the DEV <italic>UL7</italic> gene, which is a late gene, and the co-localization of its encoded protein with pUL51 in transfected DEFs, enriching our understanding of pUL7 and future research directions.</p></sec>