AUTHOR=Abbas Mohammad , Kushwaha Vandana Singh , Srivastava Kirti , Banerjee Monisha 

TITLE=Understanding Role of DNA Repair and Cytochrome p-450 Gene Polymorphisms in Cervical Cancer Patient Treated With Concomitant Chemoradiation

JOURNAL=British Journal of Biomedical Science

VOLUME=79

YEAR=2022

URL=https://www.frontierspartnerships.org/journals/british-journal-of-biomedical-science/articles/10.3389/bjbs.2021.10120

DOI=10.3389/bjbs.2021.10120

ISSN=2474-0896

ABSTRACT=<p><bold>Background:</bold> Evidences suggest that single nucleotide polymorphisms (SNPs) can be considered as potential biomarkers for disease progression and therapeutic response in cervical cancer. The present study investigated the association of <italic>CYP1A1</italic> T&gt;C (rs4646903), <italic>CYP1A1</italic> A&gt;G (rs1048943), <italic>CYP2E1</italic> T&gt;A (rs6413432), <italic>RAD51</italic> G&gt;C (rs1801320), <italic>XRCC1</italic> G&gt;A (rs25487), <italic>XRCC2</italic> G&gt;A (rs3218536) and <italic>XRCC3</italic> C&gt;T (rs861539) polymorphisms with treatment outcome of cisplatin based chemoradiation (CRT).</p><p><bold>Methods:</bold> Total 227 cervical cancer cases, treated with the same chemoradiotherapy regimen were selected for the study. Genotyping analysis was performed by PCR-restriction fragment length polymorphisms (PCR-RFLP). Treatment response was evaluated by Response Evaluation Criteria in Solid Tumors (RECIST). Association of all clinical data (responses, recurrence and survival of patients) and single nucleotide polymorphisms (SNPs) was analysed by using SPSS (version 21.0).</p><p><bold>Results:</bold> Patients with TA/AA genotype of <italic>CYP2E1</italic> T&gt;A polymorphism showed significantly poor response while those with GC/CC genotype of <italic>RAD51</italic> G&gt;C showed better response (<italic>p</italic> = 0.008, <italic>p</italic> = 0.014 respectively). Death was significantly higher in patients with GG genotypes of <italic>RAD51</italic> G&gt;C and <italic>XRCC1</italic> G&gt;A (<italic>p</italic> = 0.006, <italic>p</italic> = 0.002 respectively). Women with GC+CC genotype of <italic>RAD51</italic> G&gt;C and AG+GG of <italic>XRCC1</italic> showed better survival and also reduced risk of death (HR = 0.489, <italic>p</italic> = 0.008; HR = 0.484, <italic>p</italic> = 0.003 respectively).</p><p><bold>Conclusion:</bold> Results suggested that <italic>CYP2E1</italic> T&gt;A (rs6413432), <italic>RAD51</italic> G&gt;C (rs1801320), and <italic>XRCC1</italic> G&gt;A (rs25487) polymorphisms may be used as predictive markers for clinical outcomes in cervical cancer patients undergoing cisplatin based concomitant chemoradiotherapy.</p>