AUTHOR=Jaafar S. O. , Jaffar J. O. , Ibrahim S. A. , Jarjees K. K. 

TITLE=MicroRNA Variants miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are Linked to Endometriosis and its Severity

JOURNAL=British Journal of Biomedical Science

VOLUME=79

YEAR=2022

URL=https://www.frontierspartnerships.org/journals/british-journal-of-biomedical-science/articles/10.3389/bjbs.2021.10207

DOI=10.3389/bjbs.2021.10207

ISSN=2474-0896

ABSTRACT=<p><bold>Background:</bold> While different studies have investigated the association of SNPs with female reproductive disorders, a limited number of studies have investigated the effect of microRNAs variants in endometriosis. In this study, we evaluated the prevalence and the association of three different miRNAs variants including, miR-27a rs895819, miR-124-1 rs531564, and miR-423 rs6505162 with endometriosis to help further elucidate the importance of these variants in female reproductive disorders.</p><p><bold>Methods:</bold> A total number of 440 women (220 cases and 220 controls) were included. DNA was extracted and genotyping of the SNPs was carried out by PCR.</p><p><bold>Results:</bold> The results showed that rs895819 and rs6505162 had a significant association with endometriosis under the dominant, recessive, co-dominant, and allelic model, but rs531564 was not linked to endometriosis. Our results also imply a protective effect on endometriosis severity for AG genotype and G allele in rs895819 (<italic>p</italic> &lt; 0.001), and also for AA and AC genotypes in rs6505162 with severity in endometriosis (<italic>p</italic> &lt; 0.001). Moreover, Hardy–Weinberg equilibrium, haplotype frequency, and linkage disequilibrium between SNPs were performed.</p><p><bold>Conclusion:</bold> miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are linked to endometriosis.</p>