AUTHOR=Kafil Ali , Mohamadynejad Parisa , Moghanibashi Mehdi 

TITLE=Significant Association of DNASE1 Variable Number Tandem Repeats and Single Nucleotide Polymorphisms With Gastric Cancer

JOURNAL=British Journal of Biomedical Science

VOLUME=79

YEAR=2022

URL=https://www.frontierspartnerships.org/journals/british-journal-of-biomedical-science/articles/10.3389/bjbs.2022.10526

DOI=10.3389/bjbs.2022.10526

ISSN=2474-0896

ABSTRACT=<p><bold>Introduction:</bold> Defects in the apoptotic process are among the most important events involved in carcinogenesis, and defects in <italic>DNASE1</italic>, as one of the apoptotic machinery components, plays a role in various types of cancer. Previous studies have indicated significant differences in the <italic>DNASE1</italic> polymorphisms in different populations. We hypothesized an association of two polymorphic sites in the exon 8 and the intron 4 of the <italic>DNASE1</italic> gene with the risk of gastric cancer.</p><p><bold>Materials and Methods:</bold> The study was carried out on 120 gastric cancer patients and 120 age and sex adjusted controls using PCR and RFLP-PCR.</p><p><bold>Results:</bold> The genotype GG (rs1053874) in exon 8 of <italic>DNASE1</italic> (odds ratio [95% confidence interval]) 4.65 [2.10–10.29], <italic>p</italic> &lt; 0.001) and genotype 2/3 of variable number tandem repeat (VNTR) in the intron 4 (3.75 [1.56–9.01], <italic>p</italic> = 0.003) are both linked to gastric cancer.</p><p><bold>Conclusion:</bold> We propose that both polymorphic sites have a part to play in gastric cancer, and so may be useful diagnosis markers.</p>