AUTHOR=Miyahara Naofumi , Benazzo Alberto , Oberndorfer Felicitas , Iwasaki Akinori , Laszlo Viktoria , Döme Balasz , Hoda Mir Ali , Jaksch Peter , Klepetko Walter , Hoetzenecker Konrad 

TITLE=MiR-21 in Lung Transplant Recipients With Chronic Lung Allograft Dysfunction

JOURNAL=Transplant International

VOLUME=35

YEAR=2022

URL=https://www.frontierspartnerships.org/journals/transplant-international/articles/10.3389/ti.2021.10184

DOI=10.3389/ti.2021.10184

ISSN=1432-2277

ABSTRACT=<p><bold>Background:</bold> Micro-RNA-21 (miR-21) is a post-translational regulator involved in epithelial-to-mesenchymal transition (EMT). Since EMT is thought to contribute to chronic lung allograft dysfunction (CLAD), we aimed to characterize miR-21 expression and distinct EMT markers in CLAD.</p><p><bold>Methods:</bold> Expression of miR-21, vimentin, Notch intracellular domain (NICD) and SMAD 2/3 was investigated in explanted CLAD lungs of patients who underwent retransplantation. Circulating miR-21 was determined in collected serum samples of CLAD and matched stable recipients.</p><p><bold>Results:</bold> The frequency of miR-21 expression was higher in restrictive allograft syndrome (RAS) than in bronchiolitis obliterans syndrome (BOS) specimens (86 vs 30%, <italic>p</italic> = 0.01); Vimentin, NICD and p-SMAD 2/3 were positive in 17 (100%), 12 (71%), and 7 (42%) BOS patients and in 7 (100%), 4 (57%) and 4 (57%) RAS cases, respectively. All four markers were negative in control tissue from donor lungs. RAS patients showed a significant increase in serum concentration of miR-21 over time as compared to stable recipients (<italic>p</italic> = 0.040).</p><p><bold>Conclusion:</bold> To the best of our knowledge this is the first study highlighting the role miR-21 in CLAD. Further studies are necessary to investigate the involvement of miR-21 in the pathogenesis of CLAD and its potential as a therapeutic target.</p>