AUTHOR=Serradilla Javier , Andrés Moreno Ane Miren , Talayero Paloma , Burgos Paula , Machuca Mariana , Camps Ortega Onys , Vallejo María Teresa , Rubio Bolívar Francisco Javier , Bueno Alba , Sánchez Alba , Zambrano Cristina , De la Torre Ramos Carlos Andrés , Rodríguez Olaia , Largo Carlota , Serrano Pilar , Prieto Bozano Gerardo , Ramos Esther , López Santamaría Manuel , Stringa Pablo , Hernández Francisco TITLE=Preclinical Study of DCD and Normothermic Perfusion for Visceral Transplantation JOURNAL=Transplant International VOLUME=36 YEAR=2023 URL=https://www.frontierspartnerships.org/journals/transplant-international/articles/10.3389/ti.2023.11518 DOI=10.3389/ti.2023.11518 ISSN=1432-2277 ABSTRACT=

Considering recent clinical and experimental evidence, expectations for using DCD-derived intestines have increased considerably. However, more knowledge about DCD procedure and long-term results after intestinal transplantation (ITx) is needed. We aimed to describe in detail a DCD procedure for ITx using normothermic regional perfusion (NRP) in a preclinical model. Small bowel was obtained from pigs donors after 1 h of NRP and transplanted to the recipients. Graft Intestinal samples were obtained during the procedure and after transplantation. Ischemia-reperfusion injury (Park-Chiu score), graft rejection and transplanted intestines absorptive function were evaluated. Seven of 8 DCD procedures with NRP and ITx were successful (87.5%), with a good graft reperfusion and an excellent recovery of the recipient. The architecture of grafts was well conserved during NRP. After an initial damage of Park-chiu score of 4, all grafts recovered from ischemia-reperfusion, with no or very subtle alterations 2 days after ITx. Most recipients (71.5%) did not show signs of rejection. Only two cases demonstrated histologic signs of mild rejection 7 days after ITx. Interestingly intestinal grafts showed good absorptive capacity. The study’s results support the viability of intestinal grafts from DCD using NRP, contributing more evidence for the use of DCD for ITx.