AUTHOR=Wang Chencheng , Abadpour Shadab , Olsen Petter Angell , Wang Daxin , Stokowiec Justyna , Chera Simona , Ghila Luiza , Ræder Helge , Krauss Stefan , Aizenshtadt Aleksandra , Scholz Hanne TITLE=Glucose Concentration in Regulating Induced Pluripotent Stem Cells Differentiation Toward Insulin-Producing Cells JOURNAL=Transplant International VOLUME=37 YEAR=2024 URL=https://www.frontierspartnerships.org/journals/transplant-international/articles/10.3389/ti.2024.11900 DOI=10.3389/ti.2024.11900 ISSN=1432-2277 ABSTRACT=
The generation of insulin-producing cells from human-induced pluripotent stem cells holds great potential for diabetes modeling and treatment. However, existing protocols typically involve incubating cells with un-physiologically high concentrations of glucose, which often fail to generate fully functional IPCs. Here, we investigated the influence of high (20 mM) versus low (5.5 mM) glucose concentrations on IPCs differentiation in three hiPSC lines. In two hiPSC lines that were unable to differentiate to IPCs sufficiently, we found that high glucose during differentiation leads to a shortage of NKX6.1+ cells that have co-expression with PDX1 due to insufficient