AUTHOR=Grando Leandro , Boada Marc , Faner Rosa , Gómez-Ollés Susana , Ruiz Victoria , Bohils Marc , Albiol Joaquim , Marrero Ramses , Rosell Laia , Salinas Ivan , Ruiz Daniel , Ruiz Ángel , Rodríguez-Villar Camino , Ureña Anna , Paredes-Zapata David , Guirao Ángela , Sánchez-Etayo Gerard , Molins Laureano , Quiroga Néstor , Gómez-Brey Aroa , Michavila Xavier , Sandiumenge Alberto , Agustí Àlvar , Ramos Ricard , Bello Irene TITLE=The impact of the EVLP on the lung microbiome and its inflammatory reaction JOURNAL=Transplant International VOLUME=37 YEAR=2024 URL=https://www.frontierspartnerships.org/journals/transplant-international/articles/10.3389/ti.2024.12979 DOI=10.3389/ti.2024.12979 ISSN=1432-2277 ABSTRACT=

The pulmonary microbiome has emerged as a significant factor in respiratory health and diseases. Despite the sterile conditions maintained during ex vivo lung perfusion (EVLP), the use of antibiotics in the perfuse liquid can lead to dynamic changes in the lung microbiome. Here, we present the design of a study that aims to investigate the hypothesis that EVLP alters the lung microbiome and induces tissue inflammation. This pilot, prospective, controlled study will be conducted in two Spanish donor centers and will include seven organ donors after brain death or after controlled cardiac death. After standardized retrieval, the left lung will be preserved in cold storage and the right lung will be perfused with EVLP. Samples from bronchoalveolar lavage, perfusion and preservation solutions, and lung biopsies will be collected from both lungs and changes in lung microbiome and inflammatory response will be compared.